Dye fluorometry hydrologic measurements were performed exclusively by the analysis of multiple samples physically extracted from the water body, at multiple locations, as the dye plume was naturally dispersed.

Applications for dye tracing include, but are not be limited to: TOT (surface water and groundwater); re-aeration studies; dispersion studies; discharge measurements (by dye-dilution methods); waste buildup and flushing in estuaries and bays; wastewater retention and flushing in wetlands; combined and sanitary sewer overflows; tracing circulation and stratification in reservoirs; path tracing in karst (cavernous limestone); determination of well-drilling fluid circulation time; irrigation water uptake by plants; tagging and tracing herbicide, and tracing heated water.